Conventional Technique for Pregnancy Diagnosis & their Importance

In the large ruminants traditional methods of pregnancy diagnosis are less efficient and most of the times their accuracy is doubtful. Unlike humans, in which super specific pregnancy molecule hCG secreted by trophoblast and start coming into the urine from 15th day of fertilization. Its presence in urine could be assessing through simple antibody flourescence technique and gives fairly positive results with high success. This type of molecule is not being identified in animals yet.  Pregnancy associated glycoproteins have been identified in recent years in both large & small ruminants and their presence in blood and urine gives some way to diagnose the pregnancy. Portable ELISA kits are available in the market (not in India) to detect these molecules and may give results on the basis of Antigen - Antibody reaction. One problem persist with these molecules is that they are not so accurate for diagnosis and many times gives false positive results because of the presence of these

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